Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag? (MALS & SPR verified)

分子別名（Synonym）
FcRn,FCGRT & B2M

表達區(qū)間及表達系統(tǒng)（Source）
Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (FCM-H82W7) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1(FCGRT) & P61769-1(B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequence

蛋白結(jié)構(gòu)（Molecular Characterization）
Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) P55899-1 (FCGRT) & P61769-1 (B2M)
Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.2 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 38-40 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

標記（Biotinylation）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度（Biotin:Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.

純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）
FCGRT和B2M異二聚體蛋白（FcRn復(fù)合物）由兩個亞單位組成：p51（相當于FCGRT）和p14（相當于β-2-微球蛋白），并形成MHC類I類異二聚物。IgG受體轉(zhuǎn)運蛋白α（FCGRT）的Fc片段與單體球蛋白γ的Fc區(qū)結(jié)合，并介導(dǎo)從牛奶中攝取IgG。FCGRT在球蛋白G從母親轉(zhuǎn)移到胎兒中的可能作用。β-2-微球蛋白（B2M）是I類主要組織相容性復(fù)合物（MHC）的組成部分，參與向系統(tǒng)呈遞肽抗原。